Purification:Article Title: Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy.
Article Snippet: Purified His6-tagged autophagin-3 concentration was determined by Western blot using known amounts of His6-tagged MMP-26 as standard and analyzed with the software package Phoretix 1D Advanced version 5.10 (Nonlinear Dy1 The abbreviations used are: E1, ubiquitin-activating enzyme; E2, ubiquitin carrier protein; kb, kilobase; AEBSF, 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride; apg/aut and APG/AUT, yeast autophagy mutant and wild-type genes; Apg, expression products of APG genes; Dpa, N-3-(2,4-dinitrophenyl)-L- , -diaminopropionyl]; GABARAP, -aminobutyric acid receptor-associated protein; GATE-16, Golgi-associated ATPase enhancer of 16 kDa; MAP-LC3, microtubuleassociated protein light chain 3; Mca, 7-(methoxycoumarin-4-yl)acetyl; NEM, N-ethylmaleimide; proAPI, proaminopeptidase I; PMSF, phenylmethylsulfonyl fluoride; USP, ubiquitin-specific processing proteases. by guest on M arch 4, 2015 http://w w w .jbc.org/ D ow nloaded from namics).
Concentration Assay:Article Title: Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy.
Article Snippet: Purified His6-tagged autophagin-3 concentration was determined by Western blot using known amounts of His6-tagged MMP-26 as standard and analyzed with the software package Phoretix 1D Advanced version 5.10 (Nonlinear Dy1 The abbreviations used are: E1, ubiquitin-activating enzyme; E2, ubiquitin carrier protein; kb, kilobase; AEBSF, 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride; apg/aut and APG/AUT, yeast autophagy mutant and wild-type genes; Apg, expression products of APG genes; Dpa, N-3-(2,4-dinitrophenyl)-L- , -diaminopropionyl]; GABARAP, -aminobutyric acid receptor-associated protein; GATE-16, Golgi-associated ATPase enhancer of 16 kDa; MAP-LC3, microtubuleassociated protein light chain 3; Mca, 7-(methoxycoumarin-4-yl)acetyl; NEM, N-ethylmaleimide; proAPI, proaminopeptidase I; PMSF, phenylmethylsulfonyl fluoride; USP, ubiquitin-specific processing proteases. by guest on M arch 4, 2015 http://w w w .jbc.org/ D ow nloaded from namics).
Western Blot:Article Title: Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy.
Article Snippet: Purified His6-tagged autophagin-3 concentration was determined by Western blot using known amounts of His6-tagged MMP-26 as standard and analyzed with the software package Phoretix 1D Advanced version 5.10 (Nonlinear Dy1 The abbreviations used are: E1, ubiquitin-activating enzyme; E2, ubiquitin carrier protein; kb, kilobase; AEBSF, 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride; apg/aut and APG/AUT, yeast autophagy mutant and wild-type genes; Apg, expression products of APG genes; Dpa, N-3-(2,4-dinitrophenyl)-L- , -diaminopropionyl]; GABARAP, -aminobutyric acid receptor-associated protein; GATE-16, Golgi-associated ATPase enhancer of 16 kDa; MAP-LC3, microtubuleassociated protein light chain 3; Mca, 7-(methoxycoumarin-4-yl)acetyl; NEM, N-ethylmaleimide; proAPI, proaminopeptidase I; PMSF, phenylmethylsulfonyl fluoride; USP, ubiquitin-specific processing proteases. by guest on M arch 4, 2015 http://w w w .jbc.org/ D ow nloaded from namics).
Software:Article Title: Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy.
Article Snippet: Purified His6-tagged autophagin-3 concentration was determined by Western blot using known amounts of His6-tagged MMP-26 as standard and analyzed with the software package Phoretix 1D Advanced version 5.10 (Nonlinear Dy1 The abbreviations used are: E1, ubiquitin-activating enzyme; E2, ubiquitin carrier protein; kb, kilobase; AEBSF, 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride; apg/aut and APG/AUT, yeast autophagy mutant and wild-type genes; Apg, expression products of APG genes; Dpa, N-3-(2,4-dinitrophenyl)-L- , -diaminopropionyl]; GABARAP, -aminobutyric acid receptor-associated protein; GATE-16, Golgi-associated ATPase enhancer of 16 kDa; MAP-LC3, microtubuleassociated protein light chain 3; Mca, 7-(methoxycoumarin-4-yl)acetyl; NEM, N-ethylmaleimide; proAPI, proaminopeptidase I; PMSF, phenylmethylsulfonyl fluoride; USP, ubiquitin-specific processing proteases. by guest on M arch 4, 2015 http://w w w .jbc.org/ D ow nloaded from namics).
Ubiquitin Proteomics:Article Title: Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy.
Article Snippet: Purified His6-tagged autophagin-3 concentration was determined by Western blot using known amounts of His6-tagged MMP-26 as standard and analyzed with the software package Phoretix 1D Advanced version 5.10 (Nonlinear Dy1 The abbreviations used are: E1, ubiquitin-activating enzyme; E2, ubiquitin carrier protein; kb, kilobase; AEBSF, 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride; apg/aut and APG/AUT, yeast autophagy mutant and wild-type genes; Apg, expression products of APG genes; Dpa, N-3-(2,4-dinitrophenyl)-L- , -diaminopropionyl]; GABARAP, -aminobutyric acid receptor-associated protein; GATE-16, Golgi-associated ATPase enhancer of 16 kDa; MAP-LC3, microtubuleassociated protein light chain 3; Mca, 7-(methoxycoumarin-4-yl)acetyl; NEM, N-ethylmaleimide; proAPI, proaminopeptidase I; PMSF, phenylmethylsulfonyl fluoride; USP, ubiquitin-specific processing proteases. by guest on M arch 4, 2015 http://w w w .jbc.org/ D ow nloaded from namics).
Mutagenesis:Article Title: Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy.
Article Snippet: Purified His6-tagged autophagin-3 concentration was determined by Western blot using known amounts of His6-tagged MMP-26 as standard and analyzed with the software package Phoretix 1D Advanced version 5.10 (Nonlinear Dy1 The abbreviations used are: E1, ubiquitin-activating enzyme; E2, ubiquitin carrier protein; kb, kilobase; AEBSF, 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride; apg/aut and APG/AUT, yeast autophagy mutant and wild-type genes; Apg, expression products of APG genes; Dpa, N-3-(2,4-dinitrophenyl)-L- , -diaminopropionyl]; GABARAP, -aminobutyric acid receptor-associated protein; GATE-16, Golgi-associated ATPase enhancer of 16 kDa; MAP-LC3, microtubuleassociated protein light chain 3; Mca, 7-(methoxycoumarin-4-yl)acetyl; NEM, N-ethylmaleimide; proAPI, proaminopeptidase I; PMSF, phenylmethylsulfonyl fluoride; USP, ubiquitin-specific processing proteases. by guest on M arch 4, 2015 http://w w w .jbc.org/ D ow nloaded from namics).
Expressing:Article Title: Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy.
Article Snippet: Purified His6-tagged autophagin-3 concentration was determined by Western blot using known amounts of His6-tagged MMP-26 as standard and analyzed with the software package Phoretix 1D Advanced version 5.10 (Nonlinear Dy1 The abbreviations used are: E1, ubiquitin-activating enzyme; E2, ubiquitin carrier protein; kb, kilobase; AEBSF, 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride; apg/aut and APG/AUT, yeast autophagy mutant and wild-type genes; Apg, expression products of APG genes; Dpa, N-3-(2,4-dinitrophenyl)-L- , -diaminopropionyl]; GABARAP, -aminobutyric acid receptor-associated protein; GATE-16, Golgi-associated ATPase enhancer of 16 kDa; MAP-LC3, microtubuleassociated protein light chain 3; Mca, 7-(methoxycoumarin-4-yl)acetyl; NEM, N-ethylmaleimide; proAPI, proaminopeptidase I; PMSF, phenylmethylsulfonyl fluoride; USP, ubiquitin-specific processing proteases. by guest on M arch 4, 2015 http://w w w .jbc.org/ D ow nloaded from namics).
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